Nibbles: Chilli, Extension, Africa, Genebank, Potatoes, War, Angora

The road to Pusa Campus

For years I’ve been seeing it in writing: NBPGR, Pusa Campus, New Delhi. But the picture of it I had in my mind turned out to be quite different to the reality. The Indian Agricultural Research Institute’s Pusa Campus comprises offices, labs and staff housing, yes, but also a huge area of agricultural fields, and right in the middle of New Delhi: a rural oasis in the teeming city (clicking on the map below will take you to Google Maps).

pusa-campus1

It all started in 1934, when the Imperial Agricultural Research Institute, established in 1905 at Pusa in Bihar, was moved to Delhi following an earthquake. Hence Pusa Campus. The institute of course became the Indian Agricultural Research Institute on independence, just like its parent body, the Imperial Council of Agricultural Research, became the Indian Council of Agricultural Research (ICAR). But many of the buildings are still used. Such as the library.
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Making that haystack smaller

Germplasm collections can be very large, and that can put off potential users. What breeder really wants to screen thousands of accessions, when only a dozen might end up being useful? It’s not surprising, therefore, that people have looked for short-cuts. One approach is to make a “core collection.” You use the available data on the collection to select a sub-set which you hope will contain most of the original genetic diversity in a fraction (20%, say) of the total number of accessions. And then you evaluate that subset, rather than the whole collection, and use the results to delve back into the remaining 80% of the material, with hopefully a better chance of finding what you’re looking for.

That’s been done for lots of large collections now, with a certain amount of success in increasing their use — and usefulness. But breeders are not really satisfied. They want to shorten the odds even more. And the application of Geographic Information Systems (GIS) technology in something called the Focused Identification of Germplasm Strategy (FIGS) provides a potentially effective way of doing just that.

Jeremy described recently over at Bioversity how FIGS was used to increase the chances of finding a needle in a haystack by “start[ing] with a smaller haystack.” The haystack was 16,000 wheat accessions. The needle was resistance to powdery mildew.

It works like this: take 400 genebank samples known to have some resistance to powdery mildew and use the geographical location where they evolved and were collected to determine the environmental profile that can be associated with resistance. Then apply that profile to a further 16,089 samples with location data, using the profile as a template to identify those that were found in places that share the conditions associated with resistance. The result is a group of 1320 wheat varieties, mostly from Turkey, Iran and Afghanistan. This much more manageable subset was screened by growing them with diverse strains of powdery mildew. About 16% of the samples (211 of 1320) showed some resistance.

These varieties then moved to the next phase, molecular screening for the presence of different alleles of the Pm3 gene. More than half (111 of the 211) had Pm3 resistance, some in previously unknown forms. In the end the group isolated and identified 7 new functional alleles of the Pm3 gene. It took scientists 100 years to find the first 7 Pm3 alleles. FIGS doubled the number in a fraction of the time.

Very good. But is it always going to work? Another recent paper — in fact, a series of papers — counsels caution.

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The debunking of the genetic erosion meta-narrative continues

ResearchBlogging.orgRegular readers will be familiar with our skepticism here at the Agricultural Biodiversity Weblog about the genetic erosion meta-narrative. Not with the fact that genetic erosion has in fact occurred, and is continuing to occur, of course. Just with the notion that it has occurred everywhere, for every crop, to the overall tune of “75% over the past century.” There’s now news of a further nail in the coffin of that hoary myth.

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